human anti il 34  (R&D Systems)

Journal: Innate immunity

Article Title: Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation.

doi: 10.1177/17534259231186239

Figure Lengend Snippet: Figure 1. Generation of IL-4/IL-34/IL-10 fusion protein FP1. Molecular and functional characterization of FP1, the human IL-4/IL-34/ IL10 fusion protein. A. Schematic overview of FP1 and its amino acid sequence. The linker sequence is underlined. B. Final purification and sizing of FP1 using size exclusion gel permeation chromatography. This includes FP1 elution from HiLoad®16/600 Superdex®200 gel permeation resin equilibrated in endotoxin-free Dulbecco’s phosphate buffered saline buffer. The molecular weight of FP1 was estimated based on its elution (∼70 mls) as compared to the elution profiles of molecular mass standards including bovine thyroglobulin (670 kDa, 56.1 ml), bovine γ-globulin (158 kDa, 72.9 ml), chicken ovalbumin (44 kDa, 87.0 ml), and equine myoglobin (17 kDa, 97.7 ml) indicated with arrows (Bio-Rad # 1511901). C, D, Purity, and quality of purified FP1 was assessed by SDS-PAGE and western blots analyses using monoclonal mouse IgG1 Clone#127107 (R&D Systems; MAB2172) for hIL-10 detection and monoclonal mouse IgG1 Clone # 578416 (R&D Systems; MAB5265) for hIL-34 detection.

Article Snippet: The identity of this band was confirmed via western blot (WB) using human IL-10monoclonal mouse IgG1Clone#127107 (R&DSystems;MAB2172-100) antibody, which detects for hIL-10 and hIL-34 monoclonal mouse IgG1 Clone # 578416 (R&D Systems; MAB5265) antibody, which detects for hIL-34.

Techniques: Functional Assay, Sequencing, GPC Assay, Saline, Molecular Weight, SDS Page, Western Blot

Journal: Innate immunity

Article Title: Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation.

doi: 10.1177/17534259231186239

Figure Lengend Snippet: Figure 1. Generation of IL-4/IL-34/IL-10 fusion protein FP1. Molecular and functional characterization of FP1, the human IL-4/IL-34/ IL10 fusion protein. A. Schematic overview of FP1 and its amino acid sequence. The linker sequence is underlined. B. Final purification and sizing of FP1 using size exclusion gel permeation chromatography. This includes FP1 elution from HiLoad®16/600 Superdex®200 gel permeation resin equilibrated in endotoxin-free Dulbecco’s phosphate buffered saline buffer. The molecular weight of FP1 was estimated based on its elution (∼70 mls) as compared to the elution profiles of molecular mass standards including bovine thyroglobulin (670 kDa, 56.1 ml), bovine γ-globulin (158 kDa, 72.9 ml), chicken ovalbumin (44 kDa, 87.0 ml), and equine myoglobin (17 kDa, 97.7 ml) indicated with arrows (Bio-Rad # 1511901). C, D, Purity, and quality of purified FP1 was assessed by SDS-PAGE and western blots analyses using monoclonal mouse IgG1 Clone#127107 (R&D Systems; MAB2172) for hIL-10 detection and monoclonal mouse IgG1 Clone # 578416 (R&D Systems; MAB5265) for hIL-34 detection.

Article Snippet: The identity of this band was confirmed via western blot (WB) using human IL-10monoclonal mouse IgG1Clone#127107 (R&DSystems;MAB2172-100) antibody, which detects for hIL-10 and hIL-34 monoclonal mouse IgG1 Clone # 578416 (R&D Systems; MAB5265) antibody, which detects for hIL-34.

Techniques: Functional Assay, Sequencing, GPC Assay, Saline, Molecular Weight, SDS Page, Western Blot